Part:BBa_K1114422:Design

Level 1 MoClo Destination Vector with A and F fusion sites

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 245
  Illegal XbaI site found at 272
  Illegal SpeI site found at 1
  Illegal PstI site found at 284
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 245
  Illegal SpeI site found at 1
  Illegal PstI site found at 284
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 245
  Illegal BamHI site found at 266
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 245
  Illegal XbaI site found at 272
  Illegal SpeI site found at 1
  Illegal PstI site found at 284
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 245
  Illegal XbaI site found at 272
  Illegal SpeI site found at 1
  Illegal PstI site found at 284
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 8
  

Design Notes

This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site F on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG. Summary of modifications from original part: Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_pSB1K3.

Source

iGEM Distribution Kit